Determination of polar compounds, polymerized and oxidized triacylglycerols,
and diacylglycerols in oils and fats: results of collaborative
studies and the standardized method (Technical report)
M. C. Dobarganes1*, J. Velasco1, and A. Dieffenbacher2
1Instituto de la Grasa, Avda. Padre Garcia Tejero 4,
41012 Sevilla, Spain; 2Chemin de la Tour
Ronde 2, CH-1806 St. L�gier, Switzerland
Abstract: A description is given of the development by collaborative
study of two standardized methods for the determination of polar compounds
in oils and fats by adsorption chromatography using silica minicolumns,
and for quantification of polymerized triacylglycerols, oxidized triacylglycerols,
and diacylglycerols in polar compounds by high-performance size-exclusion
chromatography. The first procedure is sensitive, allowing savings in
time, solvents, and reagents as compared to the previous determination
(Standard Method 2.507), while the second is very rapid, giving a detailed
information on the main groups of compounds in fats and oils associated
with hydrolysis, oxidation, and thermal polymerization. Both methods
are useful for the analysis of used frying fats as well as for the analysis
of virgin or refined oils.
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* corresponding author
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