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Pure Appl. Chem.,
Vol. 71, No. 10, pp. 1983-1991, 1999
COMMISSION ON OILS, FATS AND DERIVATIVES
Determination of mono- and diacylglycerols
in edible oils and fats by high performance liquid chromatography and
evaporative light scattering detector: Results of collaborative studies
and the standardised method
(Technical Report)
D. Berner
and A. Dieffenbacher
Abstract: The development, by collaborative study, of standardised
method for the determination of mono- and diacylglycerols in vegetable
oils and fats is described. The method involves separation of mono-
and diacylglycerols by normal phase high-performance liquid-liquid chromatography
(HPLC) and evaporative light scattering detection of a solution of oil,
fat or a commercial mono- and diacylglycerol preparation in a organic
solvent.
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