34 No. 6
||Recent IUPAC technical reports and recommendations that affect the many fields of pure and applied chemistry.
See also www.iupac.org/publications/pac
Ute Resch-Genger and Paul C. DeRose
Pure and Applied Chemistry, 2012
Vol. 84, No. 8, pp. 1815-1835
Despite the widespread and increasing use of photoluminescence measuring techniques in materials science, (bio)analytical chemistry, medical diagnostics, and biotechnology, many method-inherent problems are often neglected, resulting in measurements that are unreliable and of poor quality. These problems include, e.g., the nonlinearity of the detection system, and spectral bandpass-, detector voltage- and polarization-dependent effects. Furthermore, the general need for correction of measured signals for unwanted contributions from instrument-dependent effects that are wavelength-, polarization-, and time-dependent is frequently underestimated despite the significant distortions in spectral shape and intensity that are often introduced. These luminescence-inherent drawbacks hamper the reliability of photoluminescence data, the comparability of measurements between instruments, and quantification from measured fluorescence intensities as well as the determination of relative fluorescence quantum yields.
These demands enhance the need for internationally accepted procedures for instrument calibration and instrument performance validation (IPV) in conjunction with suitable standards.
To improve the overall reliability and comparability of fluorescence measurements, the purpose of this document is to present and discuss procedures for the characterization and performance validation of photoluminescence measuring systems. Special emphasis is dedicated to steady-state measurements of photoluminescence in solution and monochromator-based systems such as are most commonly found in research-grade instrumentation. The aim of this report is also to increase the awareness for the importance of a reliable instrument characterization and to improve the reliability and comparability of measurements of photoluminescence.
last modified 31 October 2012.
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