Selection and characterization of DNAzymes with synthetically appended functionalities: A case of a synthetic RNAsea mimic
R. Ting, L. Lermer, J. Thomas, Y. Roupioz, and D. M. Perrin
Department of Chemistry, University of British Columbia,
2036 Main Mall, Vancouver, British Columbia V6T-1Z1, Canada
Abstract: We have been interested in merging synthetic nucleotide
chemistry with combinatorial selection of DNAzymes to deliver a more
complete (and complex) chemical complement to the catalytic repertoire
of nucleic acids. Thus we ask, what do modified dNTPs really bring to
nucleic acids in terms of an increased repertoire? In asking this question,
we have looked first at conditions, and more recently for reaction classes
where nucleic acids are found to be catalytically inefficient, deficient,
or at least to date, seemingly incapable of certain functions. A case
of this is M2+-independent ribophosphodiester hydrolysis
at physiological pH and low ionic strength where nucleic acids exhibit
especially low rate constants for self-cleavage and seem to be incapable
of turnover.
*Lecture presented at the symposium "Chemistry of nucleic acids", as part of the 39th IUPAC Congress and 86th Conference of the Canadian Society for Chemistry: Chemistry at the Interfaces, Ottawa, Canada, 10-15 August 2003. Other Congress presentations are published in this issue, pp. 1295-1603.
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